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noc7  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology noc7
    Noc7, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/noc7/product/Santa Cruz Biotechnology
    Average 94 stars, based on 11 article reviews
    noc7 - by Bioz Stars, 2026-03
    94/100 stars

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    ( A ) Immunoblot analysis to detect FLAG-tagged WT ADH5 and C174S mutant ADH5 that were transiently expressed in Adh5 −/− MEFs. ( B ) Measurement of GSNOR (left) and FDH (middle) activities in lysates of Adh5 −/− MEFs expressing WT ADH5 or C174S mutant ADH5. Right: The ratio of GSNOR activities to FDH activities for each ADH5 protein expressed in Adh5 −/− MEFs. Data are means ± SD ( n = 3). * P < 0.001, one-way ANOVA with Tukey’s multiple comparisons test. N.S., not significant. ( C ) Immunoblot analysis of WT and mutant ADH5 proteins expressed in Adh5 +/+ and Adh5 C174S/C174S MEFs. ( D ) Measurement of GSNOR (left) and FDH (middle) activities in lysates of Adh5 +/+ and Adh5 C174S/C174S MEFs. Right: The ratio of GSNOR activities to FDH activities for each sample. Data are means ± SD, n = 3. * P < 0.001, unpaired Student’s t test. ( E ) Viability of MEFs after <t>NOC7</t> or HM-SG treatment. Data are means ± SD, n = 4. * P < 0.001 versus Mock Adh5 −/− , # P < 0.001 versus Adh5 −/− -expressing C174S mutant, ¶ P < 0.01 versus Mock Adh5 −/− , two-way ANOVA with Tukey’s multiple comparisons test.
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    Image Search Results


    ( A ) Immunoblot analysis to detect FLAG-tagged WT ADH5 and C174S mutant ADH5 that were transiently expressed in Adh5 −/− MEFs. ( B ) Measurement of GSNOR (left) and FDH (middle) activities in lysates of Adh5 −/− MEFs expressing WT ADH5 or C174S mutant ADH5. Right: The ratio of GSNOR activities to FDH activities for each ADH5 protein expressed in Adh5 −/− MEFs. Data are means ± SD ( n = 3). * P < 0.001, one-way ANOVA with Tukey’s multiple comparisons test. N.S., not significant. ( C ) Immunoblot analysis of WT and mutant ADH5 proteins expressed in Adh5 +/+ and Adh5 C174S/C174S MEFs. ( D ) Measurement of GSNOR (left) and FDH (middle) activities in lysates of Adh5 +/+ and Adh5 C174S/C174S MEFs. Right: The ratio of GSNOR activities to FDH activities for each sample. Data are means ± SD, n = 3. * P < 0.001, unpaired Student’s t test. ( E ) Viability of MEFs after NOC7 or HM-SG treatment. Data are means ± SD, n = 4. * P < 0.001 versus Mock Adh5 −/− , # P < 0.001 versus Adh5 −/− -expressing C174S mutant, ¶ P < 0.01 versus Mock Adh5 −/− , two-way ANOVA with Tukey’s multiple comparisons test.

    Journal: Science Advances

    Article Title: Supersulfide catalysis for nitric oxide and aldehyde metabolism

    doi: 10.1126/sciadv.adg8631

    Figure Lengend Snippet: ( A ) Immunoblot analysis to detect FLAG-tagged WT ADH5 and C174S mutant ADH5 that were transiently expressed in Adh5 −/− MEFs. ( B ) Measurement of GSNOR (left) and FDH (middle) activities in lysates of Adh5 −/− MEFs expressing WT ADH5 or C174S mutant ADH5. Right: The ratio of GSNOR activities to FDH activities for each ADH5 protein expressed in Adh5 −/− MEFs. Data are means ± SD ( n = 3). * P < 0.001, one-way ANOVA with Tukey’s multiple comparisons test. N.S., not significant. ( C ) Immunoblot analysis of WT and mutant ADH5 proteins expressed in Adh5 +/+ and Adh5 C174S/C174S MEFs. ( D ) Measurement of GSNOR (left) and FDH (middle) activities in lysates of Adh5 +/+ and Adh5 C174S/C174S MEFs. Right: The ratio of GSNOR activities to FDH activities for each sample. Data are means ± SD, n = 3. * P < 0.001, unpaired Student’s t test. ( E ) Viability of MEFs after NOC7 or HM-SG treatment. Data are means ± SD, n = 4. * P < 0.001 versus Mock Adh5 −/− , # P < 0.001 versus Adh5 −/− -expressing C174S mutant, ¶ P < 0.01 versus Mock Adh5 −/− , two-way ANOVA with Tukey’s multiple comparisons test.

    Article Snippet: Cells were treated with NOC7 (0 to 3.0 mM) (Dojindo) or HM-SG (0 to 3.0 mM) in serum-free DMEM.

    Techniques: Western Blot, Mutagenesis, Expressing